Microporous Mesoporous Mater, , 1—3 : 74— Effect of pore expansion and amine functionalization of mesoporous silica on CO2 adsorption over a wide range of conditions [J]. Adsorption, , 15 3 : — Energ Fuel, , 17 92 : — Int J Greenh Gas Conl, , 3 5 — J Porous Mater, , 16 5 : — Ind Eng Chem Res, , 43 26 : — Chem Physics Letters, , 1—3 : 6—9. Adv Colloid Interfac Sci, , 1—2 : 43— Adv Funct Mater, , 16 13 : — Synthesis-structure-property relationships for hyperbranched aminosilica CO2 adsorbents [J].
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Adv Mater, , 15 22 : — Rapid functionalization of mesoporous materials: Directly dispersing metal oxides into as-prepared SBA occluded with template [J]. Then, the carbon precursor was filled into the pores of the as-prepared sSiO2 mSiO2 templates. The black products were dried in vacuum and denoted as HMCN.
For the carboxylation of HMCN, 0. This mixture was reacted for 2 hours at ambient temperature to activate the carboxyl. Subsequently, excess PEI was added and stirred for 24 hours. Next, 25 mg HA was dissolved in pH 7. Next, NHS Fourier-transform infrared spectra were measured on a Nexus spectrometer Thermo Fisher Scientific.
China at 1. The spot-effective area of the laser light source was about 0. China was employed to measure the temperature profiles during irradiation. After a predetermined time interval, 1 mL sample of the release solutions was taken from the suspension and an equal volume of fresh medium was supplemented. The cells were carefully washed three times with cold PBS following 4 hours of incubation.
In brief, HeLa cells were seeded in well plates at a density of 2. After incubation for another 2 hours, the cells were washed with PBS and incubated in fresh medium for 24 hours. Meanwhile, the untreated cells in the growth media were used as the blank control.
The cells without any treatment were used as a control. Then, all of the cells were collected and resuspended in 0. Xenograft tumor models and in vivo antitumor efficacy Female nude mice 20—25 g were provided by the Animal Center of Kunming Medical University Kunming, P.
China and were used for the animal experiments directly. China before beginning the in vivo work. The length L and width W of the tumor were measured with a caliper. The tumor size was calculated with the following formula: 2 In vivo experiments were carried out when the tumor size reached to about mm3. All mice were injected via the tail vein on day 1, 3, and 5. The weight of the mice and the tumor volume were measured every 3 days. For in vivo fluorescence imaging, mice with HeLa tumors were intravenously i.
Afterward, in vivo fluorescence imaging at different time points 2, 4, 12, 24, and 48 hours was carried out using a Lumina III in vivo imaging system PerkinElmer Inc. Histological staining Mice were sacrificed at 18 days after different treatments and the tumors were excised and weighed. After centrifugation, the amount of DOX in all samples was measured by fluorescence measurement and the pharmacokinetics analysis was performed with Win NonLin 6.
Then, the mixture was polymerized and calcinated under nitrogen to form the C—Si structure. Also, the average diameter of the nanoparticles was about nm. These results demonstrated that the obtained HMCN was suitable for drug delivery. Figure 2 Characterizations of the nanoparticles. From a viewpoint of particle size and mesoporous structure, the HMCN nanoparticles could be used as nanocarriers for drug delivery.
The zeta potential reversed from 4. Notes: A The surface charge potentials of different nanoparticles. Similarly, the photothermal performance was dependent on the irradiation power density Figure 4B.
As observed in Figure 4D , almost no noticeable attenuation was observed after five cycles of laser irradiation. Taken together, these results demonstrated that the NIR light irradiation can not only induce mild hyperthermia for PTT, but also achieve NIR-responsive drug release for the synergistic therapy of chemotherapy and PTT. Considering the fact that the tumor microenvironment is mildly acidic with a pH range of 5.
This phenomenon may be attributed to the local mild hyperthermia that could enhance the cell permeability and fluidity, thus further improving the intracellular uptake of nanoparticles. Also, the uptake of Si in laser irradiation group was as high as nearly 1. Figure 5 In vitro targeted cellular uptake and cell viability. B The mass of silicon internalized in HeLa cells after treatment with different formulations.
The cytotoxic effects of different treatments, including single chemotherapy, PTT, and synergistic chemo-PTT, were studied upon drug loading. The results showed that the viability of cells treated with different drug formulations significantly decreased, and also, a drug concentration-dependent cell inhibition was observed Figure S4. To further assess the chemo-photothermal effect, HeLa cells were co-stained with calcein AM live cells, green and PI dead cells, red after different treatments Figure 6A.J Catal, , 1 : 60— Chem Mater, , 18 17 : — In vivo antitumor efficacy Encouraged by the attractive therapeutic effect in vitro, we tried to investigate the therapeutic effects of this system in vivo.
J Am Chem Soc, , 4 : —
Conclusion: The excellent therapeutic effects demonstrated in vitro and in vivo suggested the HMCN-based nanoplatform holds potential for efficient dual-responsive targeting drug delivery and synergistic chemo-photothermal therapy. Chem Engineering Science, , 62 4 : — Microporous Mesoporous Mater, , 1—3 : 74—
Adv Mater, , 15 22 : — Adsorption of CO2 onto amine-grafted mesoporous silicas [J].
Defect and adsorbate induced infrared modes in sol-gel derived magnesium oxide nano-crystallites [J]. J Am Chem Soc, , 27 : — Ind Eng Chem Res, , 43 26 : — Subsequently, excess PEI was added and stirred for 24 hours.
A new family of mesoporous molecular sieves prepared with liquid crystal templates [J]. Notes: A The surface charge potentials of different nanoparticles. Figure 5 In vitro targeted cellular uptake and cell viability. Fourier-transform infrared spectra were measured on a Nexus spectrometer Thermo Fisher Scientific. J Phys Chem C, , 30 : — In vitro cell apoptosis assay Flow cytometry analysis was used to further evaluate chemo-photothermal effect.
After centrifugation, the amount of DOX in all samples was measured by fluorescence measurement and the pharmacokinetics analysis was performed with Win NonLin 6. For the carboxylation of HMCN, 0. Comprehensive study of pore evolution, mesostructural stability, and simultaneous surface functionalization of ordered mesoporous carbon FDU by wet oxidation as a promising adsorbent [J]. Energy Fuels, , 16 6 : —